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BIT-218

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Cell Culture and Protein Purification

Biotechnology Undergraduate Science, Math, Engineering, & Technology

Subject code

BIT

Course Number

218

4

Course Long Title

Cell Culture and Protein Purification

Academic Level

Undergraduate

Description

This course describes cell physiology and protein biochemistry and prepares students for work with recombinant protein expression systems. It introduces protein purification and characterization methods and specifically addresses bioreactor design, large-scale manufacturing, and fermentation conditions. It includes laboratory exercises on cell culture, cell quantification, cell viability, electrophoresis, western blots, immunoblots, chromatography, and cryopreservation. 3/3/0